Anti-JAK2 Antibody

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4165

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-JAK2 antibody (BM4165). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human NRK whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-JAK2 antigen affinity purified monoclonal antibody (BM4165) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for JAK2 at approximately 131 kDa. The expected band size for JAK2 is at 131 kDa.
all(3)
Figure 2. IHC analysis of JAK2 using anti-JAK2 antibody (BM4165) .
JAK2 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-JAK2 Antibody (BM4165) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IF analysis of JAK2 using anti-JAK2 antibody (BM4165) and anti-Beta Tubulin antibody (M01857-3).
JAK2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-JAK2 Antibody (BM4165) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-JAK2 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

Janus kinase 2; JTK10; JAK2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

JAK2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.

计算分子量

131 kDa

实际分子量

131 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Janus kinase 2, commanly called JAK2, is a human protein that has been implicated in signaling by members of the type II cytokine receptor family, the GM-CSF receptor family, the gp130 receptor family, and the single chain receptors. Exactly, JAK2 kinase is a member of a family of tyrosine kinases involved in cytokine receptor signaling. The JAK2 gene is mapped to 9p24.1. The JAK2 gene encodes a 1,132-amino acid protein that shares 95% sequence similarity to rat and pig Jak2. JAK2 is constitutively associated with the prolactin receptor and that it is activated and tyrosine phosphorylated upon PRL binding to the PRL receptor. JAK2, and more specifically just its intact N-terminal domain, binds to EPOR in the endoplasmic reticulum and promotes its cell surface expression. The human JAK2 is present in the nucleus of hematopoietic cells and directly phosphorylates tyr41 on histone H3.

Uniprot ID

O60674

文献引用格式

JAK2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM4165)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

实验方案>

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