Anti-Histone H3 (mono methyl K18) Antibody

Rabbit Monoclonal Antibody

说明书

筛选器： All WB IHC ICC/IF

BM4323

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-Histone H3 (mono methyl K18) antibody (BM4323). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Histone H3 (mono methyl K18) antigen affinity purified monoclonal antibody (BM4323) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Histone H3 (mono methyl K18) at approximately 17 kDa. The expected band size for Histone H3 (mono methyl K18) is at 15 kDa.
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Figure 2. IHC analysis of Histone H3 (mono methyl K18) using anti-Histone H3 (mono methyl K18) antibody (BM4323).
Histone H3 (mono methyl K18) was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of Histone H3 (mono methyl K18) using anti-Histone H3 (mono methyl K18) antibody (BM4323).
Histone H3 (mono methyl K18) was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 4. IF analysis of Histone H3 (mono methyl K18) using anti-Histone H3 (mono methyl K18) antibody (BM4323) and anti-Beta Tubulin antibody (M01857-3).
Histone H3 (mono methyl K18) was detected in an immunocytochemical section of U2OScells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Histone H3 (mono methyl K18) Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

H3/b; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone cluster 1; H3d; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

H3C1/H3C2/H3C3/H3C4/H3C6/H3C7/H3C8/H3C10/H3C11/H3C12

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Histone H3 (mono methyl K18)

计算分子量

15 kDa

实际分子量

15-17 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

Uniprot ID

P68431

文献引用格式

Histone H3 (mono methyl K18) Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM4323)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Histone H3.1

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实验步骤

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