Anti-HLA-DRA Antibody (Clone#FGI-8)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4546

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-HLA-DRA antibody (BM4546). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HLA-DRA antigen affinity purified monoclonal antibody (BM4546) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HLA-DRA at approximately 36 kDa. The expected band size for HLA-DRA is at 29 kDa.
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IHC analysis of HLA-DRA using anti-HLA-DRA antibody (BM4546) .
HLA-DRA was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (BM4546) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of HLA-DRA using anti-HLA-DRA antibody (BM4546) .
HLA-DRA was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (BM4546) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of HLA-DRA using anti-HLA-DRA antibody (BM4546) .
HLA-DRA was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (BM4546) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of HLA-DRA using anti-HLA-DRA antibody (BM4546) .
HLA-DRA was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (BM4546) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
Immunofluorescent analysis of Raji cells, using HLA-DRA Antibody.
all(7)
all(7)

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产品简介>

产品名称

Anti-HLA-DRA Antibody (Clone#FGI-8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HLA DRA; HLA DRA1; HLA-DR; HLA-DRA; MHC class II antigen DRA

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

HLA-DRA

克隆号

FGI-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human HLA-DRA

计算分子量

29 kDa

实际分子量

36 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

HLA class II histocompatibility antigen, DR alpha chain is a protein that in humans is encoded by the HLA-DRA gene. It is mapped to 6p21.32. HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Uniprot ID

P01903

文献引用格式

HLA-DRA Antibody (Clone#FGI-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4546)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

HLA class II histocompatibility antigen, DR alpha chain

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