Anti-14-3-3 alpha/beta Antibody

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP FCM

BM4752

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-14-3-3 alpha/beta antibody (BM4752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 alpha/beta antigen affinity purified monoclonal antibody (BM4752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 alpha/beta at approximately 28 kDa. The expected band size for 14-3-3 alpha/beta is at 28 kDa.
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产品简介>

产品名称

Anti-14-3-3 alpha/beta Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

14 3 3; 14 3 3 alpha; 14 3 3 protein beta; 14 3 3 protein beta alpha; 14 3 3 protein beta/alpha; YWHAB; YWHAA

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

YWHAB

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human 14-3-3 alpha + beta

计算分子量

28 kDa

实际分子量

28 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Uniprot ID

P31946

文献引用格式

14-3-3 alpha/beta Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM4752)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

14-3-3 protein beta/alpha

实验方案>

实验步骤

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