Anti-CaMKII Alpha/CAMK2A Antibody (Clone#AAFF-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP FCM

BM5046

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-CaMKII Alpha/CAMK2A antibody (BM5046). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat Hippocampus tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CaMKII Alpha/CAMK2A antigen affinity purified monoclonal antibody (BM5046) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CaMKII Alpha/CAMK2A at approximately 54 kDa. The expected band size for CaMKII Alpha/CAMK2A is at 54 kDa.
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IHC analysis of CAMK2A using anti-CAMK2A antibody (BM5046).
CAMK2A was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-CAMK2A Antibody (BM5046) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CAMK2A using anti-CAMK2A antibody (BM5046).
CAMK2A was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CAMK2A Antibody (BM5046) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-CaMKII Alpha/CAMK2A Antibody (Clone#AAFF-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CaM kinase II subunit alpha; CaMK II subunit alpha; CAMK2; CAMK2A; CAMK2B; CAMK2D; CAMK2G; CAMKA; CaMKII alpha; KIAA0968

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP, FCM

基因名称

CAMK2A

克隆号

AAFF-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human CaMKII alpha

计算分子量

54 kDa

实际分子量

54 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMKIIα), a.k.a. Ca2+/calmodulin-dependent protein kinase II alpha, is a protein kinase (i.e., an enzyme which phosphorylates proteins) that in humans is encoded by the CAMK2A gene. It is mapped to 5q32. The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.

Uniprot ID

Q9UQM7

文献引用格式

CaMKII Alpha/CAMK2A Antibody (Clone#AAFF-3) (Boster Biological Technology, Wuhan, China. Catalog#BM5046)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Calcium/calmodulin-dependent protein kinase type II subunit alpha

实验方案>

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