Anti-GATA4 Antibody (Clone#ABOB-7)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF FCM

BM5082

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-GATA4 antibody (BM5082). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GATA4 antigen affinity purified monoclonal antibody (BM5082) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GATA4 at approximately 54 kDa. The expected band size for GATA4 is at 45 kDa.
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Immunofluorescent analysis of HepG2 cells, using GATA4 Antibody .
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产品简介>

产品名称

Anti-GATA4 Antibody (Clone#ABOB-7)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GATA binding factor 4; GATA binding protein 4; GATA4; Transcription factor GATA 4

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, FCM

基因名称

GATA4

克隆号

ABOB-7

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human GATA4

计算分子量

45 kDa

实际分子量

50 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Transcription factor GATA-4 is a protein that in humans is encoded by the GATA4 gene. This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types.

Uniprot ID

P43694

文献引用格式

GATA4 Antibody (Clone#ABOB-7) (Boster Biological Technology, Wuhan, China. Catalog#BM5082)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Transcription factor GATA-4

实验方案>

实验步骤

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