Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Flow Cytometry (FCM): | 1:50 |
Western blot analysis of anti- HNRNPA1 antibody (BM5135). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Daudi whole cell lysates,
Lane 3: Molt4 whole cell lysates,
Lane 4: HL-60 whole cell lysates.
Use mouse anti- HNRNPA1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HNRNPA1 at approximately 36KD. The expected band size for HNRNPA1 is at 39KD.
Western blot analysis of anti- HNRNPA1 antibody (BM5135). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Daudi whole cell lysates,
Lane 3: Molt4 whole cell lysates,
Lane 4: HL-60 whole cell lysates.
Use mouse anti- HNRNPA1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HNRNPA1 at approximately 36KD. The expected band size for HNRNPA1 is at 39KD.