产品名称
Anti-V5-tag Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
免疫原
A synthesized peptide
成分
Purified antibody in PBS with 0.05% sodium azide and 0.349% protein stabilizer
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
文献引用格式
V5-tag Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM5421)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
推荐稀释比
Western blot (WB): | 1:500-2000 |
ImmunoPrecipitation (IP): | 1:100 |