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Anti-IBA1/AIF1 Antibody

Rabbit monoclonal antibody

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筛选器: All IHC WB ICC/IF FCM

BM5765

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. IHC analysis using anti- AIF1/IBA-1 antibody (BM5765). detected in paraffin-embedded section of human uterus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  • Figure 2. Western blot analysis of anti- AIF1/IBA-1 antibody (BM5765). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: Hela whole cell lysates,
    Lane 2: THP-1 whole cell lysates,
    Lane 3: U937 whole cell lysates,
    Lane 4: HL-60 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat thymus tissue lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse thymus tissue lysates.
    Use mouse anti- AIF1/IBA-1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AIF1/IBA-1 at approximately 17KD. The expected band size for AIF1/IBA-1 is at 17KD.

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产品简介>

产品名称
Anti-IBA1/AIF1 Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
AIF 1; AIF1; G1; IBA1; IBA 1; IBA-1; IRT 1; Protein G1
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
AIF1
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human IBA1
计算分子量
17 kDa
实际分子量
17 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Allograft inflammatory factor 1 (AIF-1), also known as ionized calcium-binding adapter molecule 1(IBA1), is a protein that in humans is encoded by the AIF1 gene. This gene encodes a protein that binds actin and calcium. And this gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.
Uniprot ID
P55008  
文献引用格式
IBA1/AIF1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM5765)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
Flow Cytometry (FCM):1:100

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    Anti-IBA1/AIF1 Antibody

    筛选器: All IHC WB ICC/IF FCM

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