Human Angiogenin/ANG ELISA Kit

96T/2480

ALS9; ANG; Angiogenin; HEL168; Ribonuclease 5; RNase 5; RNASE4; RNASE5

E.coli, Q25-P147

There is no detectable cross-reactivity with other relevant proteins.

<12 pg/ml

78 pg/ml - 5000 pg/ml

ANG

angiogenin, ribonuclease, RNase A family, 5

P03950

Human Angiogenin/ANG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0305)

Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

15-20分钟

Catalog number	Description	Quantity
EK0305-CAP	96-well plate precoated with anti-human ANG antibody	1
EK0305-ST	Lyophilized recombinant human ANG standard	10ng/tube×2
EK0305-DA	Biotinylated anti- human ANG antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	107	719	2914	100	723	2957
Standard deviation	4.81	48.17	195.23	4.6	49.16	251.34
CV(%)	4.5	6.7	6.7	4.6	6.8	8.5

Concentration(pg/ml)	0.0 78	156	313	625	1250	2500	5,000
O.D.	0.047	0.116	0.169	0.265	0.506	0.927	1.534	2.375

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h