产品名称
Bovine BMP5 ELISA Kit
指标别名
BMP 5; BMP5; BMP-5; bone morphogenetic protein 5
免疫原
Expression system for standard: NSO; Immunogen sequence: A317-H454
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
Bone morphogenetic protein 5
文献引用格式
Bovine BMP5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0310-BV)
背景资料
Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on Bovine chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0310-BV-CAP | Anti-Human BMP5 Pre-coated 96-well strip microplate | 1 |
EK0310-BV-ST | Human BMP5 Standard | 10ng/tube×2 |
EK0310-BV-DA | Human BMP5 Biotinylated antibody (100x) | 100µl |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(ng/ml) | 1.23 | 3.46 | 6.92 | 1.38 | 3.57 | 6.85 |
Standard deviation | 0.059 | 0.180 | 0.388 | 0.073 | 0.203 | 0.425 |
CV(%) | 4.8 | 5.2 | 5.6 | 5.3 | 5.7 | 6.2 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.056 | 0.099 | 0.116 | 0.201 | 0.348 | 0.634 | 1.135 | 1.946 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Bone Tissue