Human BMP2 ELISA Kit

说明书

EK0311

96T ￥2480
货期：现货

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Human BMP-2 ELISA Kit standard curve
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产品简介>

产品名称

Human BMP2 ELISA Kit

规格/价格

96T/2480

指标别名

BMP-2; BMP 2; BMP 2A; BMP2; BMP2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A

免疫原

CHO, Q283-R396

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<2 pg/ml

检测范围

31.2 pg/ml - 2000 pg/ml

基因名称

BMP2

基因名全称

bone morphogenetic protein 2

Uniprot ID

P12643

文献引用格式

Human BMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0311)

背景资料

Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK0311-CAP	96-well plate precoated with anti- human BMP-2 antibody	1
EK0311-ST	Lyophilized recombinant human BMP-2 standard	10ng/tube×2
EK0311-DA	Biotinylated anti- human BMP-2 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	55	250	1005	59	255	1061
Standard deviation	4.29	13.25	70.35	4.66	15.55	83.81
CV(%)	7.8	5.3	7	7.9	6.1	7.9

典型数据示例

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.005	0.034	0.059	0.119	0.277	0.680	1.642	2.417

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human BMP2 ELISA Kit

EK0311

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

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Human BMP2 ELISA Kit

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