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Rat ICAM1 ELISA Kit

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EK0372

  • 96T ¥2280
  • 货期: 现货

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产品名称
Rat ICAM1 ELISA Kit
规格/价格
96T/2280
指标别名
BB2; CD54; ICAM 1; ICAM1; ICAM-1; P3.58; Ly 47; MALA 2
免疫原
Expression system for standard: NS0; Immunogen sequence: Q28-T493
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2pg/ml-2000pg/ml
基因名称
ICAM1
基因名全称
intercellular adhesion molecule 1
Uniprot ID
Q00238  
文献引用格式
Rat ICAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0372)
背景资料
Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds.
试剂盒内容
Catalog numberDescriptionQuantity
EK0372-CAP96-well plate precoated with anti- rat ICAM-1 antibody1
EK0372-STLyophilized recombinant rat ICAM-1 standard10ng/tube×2
EK0372-DABiotinylated anti- rat ICAM-1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)5123178751242855
Standard deviation3.6714.5542.494.2316.2151.3
CV(%)7.2%6.3%5.4%8.3%6.7%6%
典型数据示例
Concentration(pg/ml)031.262.512525050010002000
O.D.0.0300.0830.1290.2350.4370.8451.3782.021
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

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