指标别名
IL 2; IL2; IL-2; interleukin 2; T cell growth factor; TCGF
免疫原
Expression system for standard: E.coli; Immunogen sequence: A21-Q169
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0398)
背景资料
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0398-CAP | 96-well plate precoated with anti- mouse IL-2 antibody | 1 |
EK0398-ST | Lyophilized recombinant mouse IL-2 standard | 10ng/tubex2 |
EK0398-DA | Biotinylated anti- mouse IL-2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 37 | 156 | 467 | 40 | 166 | 452 |
Standard deviation | 2.1 | 8.58 | 35.95 | 2.6 | 10.62 | 37.06 |
CV(%) | 5.7% | 5.5% | 7.7% | 6.5% | 6.4% | 8.2% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.067 | 0.111 | 0.166 | 0.288 | 0.453 | 1.080 | 1.669 | 2.221 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)