Mouse M-CSF/CSF1 ELISA Kit

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EK0445

96T ￥2480
货期：现货

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Mouse M-CSF ELISA Kit standard curve
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产品简介>

产品名称

Mouse M-CSF/CSF1 ELISA Kit

规格/价格

96T/2480

指标别名

CSF 1; CSF1; Lanimostim; M CSF; MCSF; M-CSF

免疫原

Expression system for standard: E.coli; Immunogen sequence: K33-E262

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<1pg/ml

检测范围

31.2pg/ml-2000pg/ml

基因名称

Csf1

基因名全称

Macrophage colony-stimulating factor 1

Uniprot ID

P07141

文献引用格式

Mouse M-CSF/CSF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0445)

背景资料

M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.

试剂盒内容

Catalog number	Description	Quantity
EK0445-CAP	96-well plate precoated with anti- mouse M-CSF antibody	1
EK0445-ST	Lyophilized recombinant mouse M-CSF standard	10ng/tubex2
EK0445-DA	Biotinylated anti- mouse M-CSF antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	40	160	459	36	158	501
Standard deviation	1.68	11.2	21.57	1.9	13.58	30.56
CV(%)	4.2%	7%	4.7%	5.3%	8.6%	6.1%

典型数据示例

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.028	0.156	0.239	0.343	0.650	1.278	1.768	2.547

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Mouse M-CSF/CSF1 ELISA Kit

EK0445

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

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EK0444

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Mouse M-CSF/CSF1 ELISA Kit

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