产品名称
Human MMP13 ELISA Kit
指标别名
CLG3; Matrix metalloproteinase 13; MMP 13; MMP13
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
matrix metallopeptidase 13 (collagenase 3)
文献引用格式
Human MMP13 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0468)
背景资料
Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0468-CAP | 96-well plate precoated with anti- human MMP13 antibody | 1 |
EK0468-ST | Lyophilized recombinant human MMP13 standard | 10ng/tubex2 |
EK0468-DA | Biotinylated anti- human MMP13 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 219 | 1742 | 4482 | 203 | 1816 | 4413 |
Standard deviation | 14.01 | 116.71 | 313.74 | 15.22 | 145.28 | 357.45 |
CV(%) | 6.4 | 6.7 | 7 | 7.5 | 8 | 8.1 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.083 | 0.185 | 0.287 | 0.494 | 0.794 | 1.178 | 1.483 | 1.886 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)