产品名称
bEnd.3 Human HNMT ELISA Kit
指标别名
histamine N methyltransferase; HMT; HNMT; HNMT S1; HNMT S2
免疫原
Expression system for standard: E.coli; Immunogen sequence: A2-A292
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
histamine N-methyltransferase
文献引用格式
Human HNMT ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2216)
背景资料
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK2216-CAP | 96-well plate precoated with anti- Human HNMT ELISA Kit A antibody | 1 |
| EK2216-ST | Lyophilized recombinant Human HNMT ELISA Kit A standard | 1ng/tube×2 |
| EK2216 -DA | Biotinylated anti- Human HNMT ELISA Kit A antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 113 | 684 | 1463 | 110 | 702 | 1532 |
| Standard deviation | 4.41 | 34.88 | 70.22 | 5.72 | 31.59 | 78.13 |
| CV(%) | 3.9 | 5.1 | 4.8 | 5.2 | 4.5 | 5.1 |
典型数据示例
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.049 | 0.072 | 0.119 | 0.198 | 0.421 | 0.665 | 1.278 | 2.012 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)
蛋白名全称
Histamine N-methyltransferase