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Mouse E-selectin/CD62E/SELE ELISA Kit

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EK0502

  • 96T ¥2280
  • 货期: 现货

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产品简介>

产品名称
Mouse E-selectin/CD62E/SELE ELISA Kit
规格/价格
96T/2280
指标别名
CD62E; ELAM; ELAM 1; ELAM1; ESEL; E-selectin; E-selectin /CD62E; LECAM2; SELE; selectin E
免疫原
Expression system for standard: NS0; Immunogen sequence: W22-P557
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<10pg/ml
检测范围
156pg/ml-10000pg/ml
基因名称
SELE
基因名全称
E-selectin
Uniprot ID
Q00690  
文献引用格式
Mouse E-selectin/CD62E/SELE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0502)
背景资料
E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.
试剂盒内容
Catalog numberDescriptionQuantity
EK0502-CAP96-well plate precoated with anti- mouse sE-Selectin antibody1
EK0502-STLyophilized recombinant mouse sE-Selectin standard10ng/tubex2
EK0502-DABiotinylated anti- mouse sE-Selectin antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2721580432927714514721
Standard deviation11.96105.86186.1416.62108.82250.21
CV(%)4.4%6.7%4.3%6%7.5%5.3%
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0130.0410.0800.2010.3680.7511.4742.156
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, citrate)
实验时长
3.5h

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    Mouse E-selectin/CD62E/SELE ELISA Kit

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