产品名称
Mouse E-selectin/CD62E/SELE ELISA Kit
指标别名
CD62E; ELAM; ELAM 1; ELAM1; ESEL; E-selectin; E-selectin /CD62E; LECAM2; SELE; selectin E
免疫原
Expression system for standard: NS0; Immunogen sequence: W22-P557
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse E-selectin/CD62E/SELE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0502)
背景资料
E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0502-CAP | 96-well plate precoated with anti- mouse sE-Selectin antibody | 1 |
EK0502-ST | Lyophilized recombinant mouse sE-Selectin standard | 10ng/tubex2 |
EK0502-DA | Biotinylated anti- mouse sE-Selectin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 272 | 1580 | 4329 | 277 | 1451 | 4721 |
Standard deviation | 11.96 | 105.86 | 186.14 | 16.62 | 108.82 | 250.21 |
CV(%) | 4.4% | 6.7% | 4.3% | 6% | 7.5% | 5.3% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.013 | 0.041 | 0.080 | 0.201 | 0.368 | 0.751 | 1.474 | 2.156 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, citrate)