Horse equine TGF Alpha/TGFA ELISA Kit

96T/2480

EGF like TGF; ETGF; TFGA; TGF alpha; TGF type 1; TGFA

Expression system for standard: E.coli; Immunogen sequence: V40-A89

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

15.6pg/ml-1000pg/ml

TGFA

Protransforming growth factor alpha

P01135  

Horse equine TGF Alpha/TGFA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0511-EQ)

Transforming growth factor alpha(TGF-alpha) is upregulated in some horse equine cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to horse equine chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic horse equine TGF-alpha showed similar immunoreactivity when compared with horse equine TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

25-30分钟

Catalog number	Description	Quantity
EK0511-EQ-CAP	Anti-Equine TGFA Pre-coated 96-well strip microplate	1
EK0511-EQ-ST	Equine TGFA Standard	10ng/tube×2
EK0511-EQ-DA	Equine TGFA Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	118	225	516	126	238	509
Standard deviation	5.43	12.83	16.5	8.95	14.28	27
CV (%)	4.6	5.7	3.2	7.1	6	5.3

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.102	0.199	0.291	0.443	0.711	1.156	1.676	2.315

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

equine

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and horse equine milk