产品名称
Human TNFR1/TNFRSF1A ELISA Kit
指标别名
CD120a; FPF; p55; p55 R; p60; TBP1; TBPI; TNF R; TNF R I; TNF R1; TNF R55; TNF Receptor 1; TNF Receptor I; TNF RI; TNFAR; TNFR I; TNFR1; TNFR55; TNFR60; TNFRSF1A
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8 pg/ml - 500 pg/ml
基因名全称
tumor necrosis factor receptor superfamily, member 1A
文献引用格式
Human TNFR1/TNFRSF1A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0528)
背景资料
TNFsRI(also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors(TNF). In contrast, the p60(TNFRSF1A) and p80(TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain(PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL(TNFRSF10A), CD40, and FAS(TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor(the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0528-CAP | 96-well plate precoated with anti- human TNFsR I antibody | 1 |
| EK0528-ST | Lyophilized recombinant human TNFsR I standard | 10ng/tubex2 |
| EK0528-DA | Biotinylated anti- human TNFsR I antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 10 | 54 | 219 | 10 | 59 | 203 |
| Standard deviation | 0.55 | 3.99 | 13.14 | 0.58 | 4.48 | 13.39 |
| CV(%) | 5.5 | 7.4 | 6 | 5.8 | 7.6 | 6.6 |
典型数据示例
| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.024 | 0.092 | 0.149 | 0.249 | 0.499 | 0.871 | 1.574 | 2.361 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine
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