Human uPA/Urokinase/PLAU ELISA Kit

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EK0535

96T ￥2480
货期：现货

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Human uPA/PLAU ELISA Kit standard curve
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产品名称

Human uPA/Urokinase/PLAU ELISA Kit

规格/价格

96T/2480

指标别名

ATF; PLAU; U plasminogen activator; uPA; uPA/Urokinase; URK

免疫原

NSO, M1-L431

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<5 pg/ml

检测范围

62.5 pg/ml - 4000 pg/ml

基因名称

PLAU

基因名全称

plasminogen activator, urokinase

Uniprot ID

P00749

文献引用格式

Human uPA/Urokinase/PLAU ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0535)

背景资料

Plasminogen activator,urokinase(PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease(AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell(VSMC), uPA stimulates migration via the uPA receptor(uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase(PI3-K).

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK0535-CAP	96-well plate precoated with anti- human uPA antibody	1
EK0535-ST	Lyophilized recombinant human uPA standard	10ng/tubex2
EK0535-DA	Biotinylated anti- human uPA antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	195	583	1373	211	536	1493
Standard deviation	8.77	39.06	74.14	12.87	44.48	103.01
CV(%)	4.5	6.7	5.4	6.1	8.3	6.9

典型数据示例

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.002	0.080	0.174	0.283	0.564	0.853	1.408	2.104

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human uPA/Urokinase/PLAU ELISA Kit

EK0535

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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Human uPA/Urokinase/PLAU ELISA Kit

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