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Mouse VEGF/VEGFA ELISA Kit

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EK0541

  • 96T ¥2280
  • 货期: 现货

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产品名称
Mouse VEGF/VEGFA ELISA Kit
规格/价格
96T/2280
指标别名
L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF
免疫原
Expression system for standard: NS0; Immunogen sequence: A27-R190
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<2pg/ml
检测范围
15.6pg/ml-1000pg/ml
基因名称
VEGFA
基因名全称
Vascular endothelial growth factor A
Uniprot ID
Q00731  
文献引用格式
Mouse VEGF/VEGFA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0541)
背景资料
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.
试剂盒内容
Catalog numberDescriptionQuantity
EK0541-CAP96-well plate precoated with anti- mouse VEGF antibody1
EK0541-STLyophilized recombinant mouse VEGF standard10ng/tubex2
EK0541-DABiotinylated anti- mouse VEGF antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)4017953840171574
Standard deviation1.929.4836.582.69.5748.79
CV(%)4.8%5.3%6.8%6.5%5.6%8.5%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0080.0850.1650.3070.5921.1221.9342.432
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)
实验时长
3.5h

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