指标别名
ACE; ACE1; Angiotensin converting enzyme; CD143; DCP; DCP1; Dipeptidyl carboxypeptidase I; Kininase II; MVCD3
免疫原
Expression system for standard: NS0; Immunogen sequence: L35-Q1264
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Angiotensin-converting enzyme
文献引用格式
Mouse ACE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0558)
背景资料
Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0976-CAP | 96-well plate precoated with anti- mouse ACE antibody | 1 |
| EK0976-ST | Lyophilized recombinant mouse ACE standard | 10ng/tubex2 |
| EK0976-DA | Biotinylated anti- mouse ACE antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 157 | 733 | 3455 | 155 | 795 | 3149 |
| Standard deviation | 6.9 | 40.31 | 272.94 | 7.59 | 46.11 | 286.55 |
| CV(%) | 4.4% | 5.5% | 7.9% | 4.9% | 5.8% | 9.1% |
典型数据示例
| Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.084 | 0.128 | 0.178 | 0.253 | 0.444 | 0.658 | 1.511 | 2.327 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)
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