产品名称
Human TNF Beta/LTA ELISA Kit
指标别名
LT; LT alpha; LTA; Lymphotoxin alpha; TNF beta; TNFB; TNF-beta; TNFSF1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
文献引用格式
Human TNF Beta/LTA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0584)
背景资料
Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0584-CAP | 96-well plate precoated with anti- human TNF beta antibody | 1 |
EK0584-ST | Lyophilized recombinant human TNF beta standard | 10ng/tubex2 |
EK0584-DA | Biotinylated anti- human TNF beta antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 165 | 487 | 1791 | 179 | 521 | 1896 |
Standard deviation | 11.71 | 32.14 | 127.16 | 13.06 | 38.55 | 157.36 |
CV(%) | 7.1 | 6.6 | 7.1 | 7.3 | 7.4 | 8.3 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.030 | 0.119 | 0.203 | 0.344 | 0.621 | 1.040 | 1.657 | 2.091 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)