Human CD105/Endoglin/ENG ELISA Kit

96T/2480

CD105; END; Endoglin; ENG; HHT1; ORW; ORW1

CHO, E26-G586

There is no detectable cross-reactivity with other relevant proteins.

<15 pg/ml

156 pg/ml - 10000 pg/ml

ENG

endoglin

P17813  

Human CD105/Endoglin/ENG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0644)

CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta(TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.

15-20分钟

Catalog number	Description	Quantity
EK0644-CAP	96-well plate precoated with anti- human CD105 antibody	1
EK0644-ST	Lyophilized recombinant human CD105 standard	10ng/tubex2
EK0644-DA	Biotinylated anti- human CD105 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	312	1384	4821	316	1275	4955
Standard deviation	16.22	74.73	318.18	17.38	72.67	386.49
CV(%)	5.2	5.4	6.6	5.5	5.7	7.8

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.011	0.258	0.471	0.766	1.312	1.658	2.122	2.480

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

human

3.5h

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