指标别名
AXL; AXL oncogene; AXL receptor tyrosine kinase; JTK11; UFO
免疫原
Expression system for standard: NS0; Immunogen sequence: H20-P443
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tyrosine-protein kinase receptor UFO
文献引用格式
Mouse AXL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0660)
背景资料
Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0660-CAP | 96-well plate precoated with anti- mouse AXL antibody | 1 |
EK0660-ST | Lyophilized recombinant mouse AXL standard | 10ng/tubex2 |
EK0660-DA | Biotinylated anti- mouse AXL antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 53 | 280 | 992 | 48 | 266 | 1046 |
Standard deviation | 3.86 | 14.84 | 66.46 | 3.55 | 14.09 | 81.58 |
CV(%) | 7.3% | 5.3% | 6.7% | 7.4% | 5.3% | 7.8% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.063 | 0.090 | 0.135 | 0.201 | 0.345 | 0.655 | 1.155 | 2.066 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)