Mouse AXL ELISA Kit

96T/2480

AXL; AXL oncogene; AXL receptor tyrosine kinase; JTK11; UFO

Expression system for standard: NS0; Immunogen sequence: H20-P443

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

31.2pg/ml-2000pg/ml

AXL

Tyrosine-protein kinase receptor UFO

Q00993

Mouse AXL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0660)

Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.

Catalog number	Description	Quantity
EK0660-CAP	96-well plate precoated with anti- mouse AXL antibody	1
EK0660-ST	Lyophilized recombinant mouse AXL standard	10ng/tubex2
EK0660-DA	Biotinylated anti- mouse AXL antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	53	280	992	48	266	1046
Standard deviation	3.86	14.84	66.46	3.55	14.09	81.58
CV(%)	7.3%	5.3%	6.7%	7.4%	5.3%	7.8%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.063	0.090	0.135	0.201	0.345	0.655	1.155	2.066

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h