产品名称
Human N-Cadherin/CDH2 ELISA Kit
指标别名
Cadherin 2; CD325; CDH2; CDHN; CDw325; N cadherin; NCAD; N-cadherin; Neural cadherin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
cadherin 2, type 1, N-cadherin (neuronal)
文献引用格式
Human N-Cadherin/CDH2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0669)
背景资料
Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0669-CAP | 96-well plate precoated with anti- human Cadherin-2 antibody | 1 |
EK0669-ST | Lyophilized recombinant human Cadherin-2 standard | 10ng/tubex2 |
EK0669-DA | Biotinylated anti- human Cadherin-2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 223 | 1172 | 4928 | 308 | 1190 | 4953 |
Standard deviation | 13.16 | 50.40 | 202.05 | 20.33 | 69.02 | 376.43 |
CV(%) | 5.9 | 4.3 | 4.1 | 6.6 | 5.8 | 7.6 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.019 | 0.121 | 0.214 | 0.384 | 0.732 | 1.020 | 1.810 | 2.121 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)