产品名称
Human Cathepsin D/CTSD ELISA Kit
指标别名
cathepsin D; CLN10; CPSD; CTSD
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human Cathepsin D/CTSD ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0672)
背景资料
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0672-CAP | 96-well plate precoated with anti- human Cathepsin D antibody | 1 |
EK0672-ST | Lyophilized recombinant human Cathepsin D standard | 10ng/tubex2 |
EK0672-DA | Biotinylated anti- human Cathepsin D antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 189 | 1630 | 5138 | 181 | 1712 | 5150 |
Standard deviation | 13.6 | 91.28 | 339.1 | 13.75 | 111.28 | 427.45 |
CV(%) | 7.2 | 5.6 | 6.6 | 7.6 | 6.5 | 8.3 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.000 | 0.075 | 0.173 | 0.287 | 0.525 | 0.941 | 1.542 | 2.314 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)