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Human Cathepsin D/CTSD ELISA Kit

说明书

EK0672

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human Cathepsin D/CTSD ELISA Kit
规格/价格
96T/2480
指标别名
cathepsin D; CLN10; CPSD; CTSD
免疫原
NSO, L21-L412
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<10 pg/ml
检测范围
156 pg/ml - 10000 pg/ml
基因名称
CTSD
基因名全称
cathepsin D
Uniprot ID
P07339  
文献引用格式
Human Cathepsin D/CTSD ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0672)
背景资料
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0672-CAP96-well plate precoated with anti- human Cathepsin D antibody1
EK0672-STLyophilized recombinant human Cathepsin D standard10ng/tubex2
EK0672-DABiotinylated anti- human Cathepsin D antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)1891630513818117125150
Standard deviation13.691.28339.113.75111.28427.45
CV(%)7.25.66.67.66.58.3
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0000.0750.1730.2870.5250.9411.5422.314
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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