Catalog number | Description | Quantity |
---|---|---|
EK0680-CAP | 96-well plate precoated with anti- human CA1 antibody | 1 |
EK0680-ST | Lyophilized recombinant human CA1 standard | 20ng/tube×2 |
EK0680-DA | Biotinylated anti- human CA1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 452 | 2590 | 9786 | 584 | 2656 | 9986 |
Standard deviation | 18.98 | 157.99 | 636.09 | 31.54 | 114.21 | 559.22 |
CV(%) | 4.2 | 6.1 | 6.5 | 5.4 | 4.3 | 5.6 |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.046 | 0.110 | 0.159 | 0.293 | 0.478 | 0.821 | 1.386 | 1.833 |
Human CA1 ELISA Kitstandard curve
Human CA1 ELISA Kitstandard curve