Human MIP-4/CCL18 ELISA Kit

96T/2480

AMAC 1; AMAC1; C C motif chemokine 18; CC chemokine PARC; CCL18; CKb7; DC CK1; DCCK1; Dendritic cell chemokine 1; MIP 4; MIP4; PARC; SCYA18; Small inducible cytokine A18

E.coli, A21-A89

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

7.8 pg/ml - 500 pg/ml

CCL18

chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)

P55774

Human MIP-4/CCL18 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0686)

Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

25-30分钟

Catalog number	Description	Quantity
EK0686-CAP	96-well plate precoated with anti- human CCL18 antibody	1
EK0686-ST	Lyophilized recombinant human CCL18 standard	10ng/tubex2
EK0686-DA	Biotinylated anti- human CCL18 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	18	49	269	18	52	279
Standard deviation	0.99	3.52	20.17	1.29	4.62	22.59
CV(%)	5.5	7.2	7.5	7.2	8.9	8.1

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.006	0.045	0.075	0.186	0.377	0.754	1.505	2.145

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum

3.5h