产品名称
Mouse CD127/IL7R ELISA Kit
指标别名
CD127; CDW127; IL 7 receptor subunit alpha; IL 7R alpha; IL 7R subunit alpha; IL 7RA; IL7R; IL-7R; IL 7R; IL7RA; ILRA; interleukin 7 receptor
免疫原
Expression system for standard: NS0; Immunogen sequence: E21-D239
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Interleukin-7 receptor subunit alpha
文献引用格式
Mouse CD127/IL7R ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0714)
背景资料
The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0714-CAP | 96-well plate precoated with anti- mouse IL7R antibody | 1 |
EK0714-ST | Lyophilized recombinant mouse IL7R standard | 20ng/tube×2 |
EK0714-DA | Biotinylated anti- mouse IL7R antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 487 | 1693 | 8518 | 442 | 1527 | 9039 |
Standard deviation | 24.83 | 82.95 | 570.7 | 23.86 | 97.72 | 759.27 |
CV(%) | 5.1% | 4.9% | 6.7% | 5.4% | 6.4% | 8.4% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.005 | 0.154 | 0.229 | 0.420 | 0.751 | 1.177 | 1.526 | 1.976 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum