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Mouse PF4/CXCL4 ELISA Kit

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EK0727

  • 96T ¥2480
  • 货期: 现货

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产品名称
Mouse PF4/CXCL4 ELISA Kit
规格/价格
96T/2480
指标别名
platelet factor 4; CXCL4; PF-4; SCYB4; PF4
免疫原
Expression system for standard: E.coli; Immunogen sequence: V30-S105
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<1pg/ml
检测范围
15.6pg/ml-1000pg/ml
基因名称
CXCL4
基因名全称
Platelet factor 4
Uniprot ID
Q9Z126  
文献引用格式
Mouse PF4/CXCL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0727)
背景资料
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
试剂盒内容
Catalog numberDescriptionQuantity
EK0727-CAP96-well plate precoated with anti- mouse CXCL4 antibody1
EK0727-STLyophilized recombinant mouse CXCL4 standard10ng/tube×2
EK0727-DABiotinylated anti- mouse CXCL4 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3226342533253425
Standard deviation1.6911.0428.052.2715.1830.6
CV(%)5.3%4.2%6.6%6.9%6%7.2%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0300.0560.0770.1150.2450.4091.0832.040
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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