产品名称
Human MIG/CXCL9 ELISA Kit
指标别名
C X C motif chemokine 9; CMK; crg 10; CXCL9; Humig; MIG; MIG; CXCL9; SCYB9; Small inducible cytokine B9
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
chemokine (C-X-C motif) ligand 9
文献引用格式
Human MIG/CXCL9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0732)
背景资料
Chemokine(C-X-C motif) ligand 9(CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon(MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0732-CAP | 96-well plate precoated with anti- human CXCL9 antibody | 1 |
EK0732-ST | Lyophilized recombinant human CXCL9 standard | 10ng/tube×2 |
EK0732-DA | Biotinylated anti- human CXCL9 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 84 | 277 | 773 | 77 | 272 | 823 |
Standard deviation | 6.55 | 11.63 | 57.2 | 6.16 | 11.69 | 60.9 |
CV(%) | 7.8 | 4.2 | 7.4 | 8 | 4.3 | 7.4 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.023 | 0.076 | 0.140 | 0.231 | 0.432 | 0.881 | 1.581 | 2.223 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)