Human CXCL16 ELISA Kit

96T/2480

C X C motif chemokine 16; CXCL16; CXCLG16; SCYB16; Small inducible cytokine B16; SR PSOX; SRPSOX; Transmembrane chemokine CXCL16

E.coli, N49-P137

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

93.7 pg/ml - 6000 pg/ml

CXCL16

chemokine (C-X-C motif) ligand 16

Q9H2A7  

Human CXCL16 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0741)

Chemokine(C-X-C motif) ligand 16(CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines(with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T(NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16(CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

25-30分钟

Catalog number	Description	Quantity
EK0741-CAP	96-well plate precoated with anti- human CXCL16 antibody	1
EK0741-ST	Lyophilized recombinant human CXCL16 standard	10ng/tube×2
EK0741-DA	Biotinylated anti- human CXCL16 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	122	725	2351	133	696	2150
Standard deviation	9.15	55.82	185.72	10.24	55.68	197.8
CV(%)	7.5	7.7	7.9	7.7	8	9.2

Concentration(pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.055	0.204	0.358	0.787	0.906	1.686	2.029	2.120

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h