产品名称
Human Decorin/DCN ELISA Kit
指标别名
Bone proteoglycan II; CSCD; DCN; Decorin; DSPG2; PG S2; PG40; PGII; PGS2; SLRR1B
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
文献引用格式
Human Decorin/DCN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0749)
背景资料
Decorin is a proteoglycan on average 90 - 140 kilodaltons(kD) in size.It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0749-CAP | 96-well plate precoated with anti- human Decorin antibody | 1 |
EK0749-ST | Lyophilized recombinant human Decorin standard | 10ng/tube×2 |
EK0749-DA | Biotinylated anti- human Decorin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 60 | 247 | 1001 | 56 | 270 | 1067 |
Standard deviation | 4.56 | 16.54 | 70.07 | 4.31 | 20.25 | 88.56 |
CV(%) | 7.6 | 6.7 | 7 | 7.7 | 7.5 | 8.3 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.003 | 0.067 | 0.111 | 0.218 | 0.407 | 0.820 | 1.339 | 2.191 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)