产品名称
Human HBEGF ELISA Kit
指标别名
HBEGF; DTR; DTS; HEGFL
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8 pg/ml - 500 pg/ml
基因名全称
heparin-binding EGF-like growth factor
文献引用格式
Human HBEGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0770)
背景资料
HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0770-CAP | 96-well plate precoated with anti- human HBEGF antibody | 1 |
EK0770-ST | Lyophilized recombinant human HBEGF standard | 10ng/tube×2 |
EK0770-DA | Biotinylated anti- human HBEGF antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 16 | 77 | 282 | 15 | 70 | 280 |
Standard deviation | 1 | 3.77 | 11.56 | 1.05 | 4.2 | 11.76 |
CV(%) | 6.3 | 4.9 | 4.1 | 7 | 6 | 4.2 |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.029 | 0.057 | 0.083 | 0.150 | 0.295 | 0.588 | 1.496 | 2.295 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)