产品名称
Mouse MBL2 ELISA Kit
指标别名
COLEC1; Collectin 1; HSMBPC; Mannan binding protein; Mannose binding lectin; Mannose binding protein C; MBL; MBL2; MBP; MBP C; MBP-C; MBP1
免疫原
Expression system for standard: NS0; Immunogen sequence: E19-D244
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Mannose-binding protein C
文献引用格式
Mouse MBL2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0806)
背景资料
MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0806-CAP | 96-well plate precoated with anti- mouse MBL2 antibody | 1 |
EK0806-ST | Lyophilized recombinant mouse MBL2 standard | 20ng/tube×2 |
EK0806-DA | Biotinylated anti- mouse MBL2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 656 | 1840 | 9232 | 637 | 1954 | 8997 |
Standard deviation | 32.8 | 84.64 | 618.54 | 42.04 | 111.37 | 665.77 |
CV(%) | 5% | 4.6% | 6.7% | 6.6% | 5.7% | 7.4% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.107 | 0.168 | 0.231 | 0.353 | 0.622 | 1.058 | 1.865 | 2.705 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)