产品名称
Human PSA/KLK3 ELISA Kit
指标别名
APS; Gamma seminoprotein; hK3; Kallikrein 3; kallikrein related peptidase 3; KLK2A1; KLK3; KLK3/PSA; P 30 antigen; Prostate specific antigen; PSA; Semenogelase; Seminin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
kallikrein-related peptidase 3
文献引用格式
Human PSA/KLK3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0816)
背景资料
Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0816-CAP | 96-well plate precoated with anti- Human Kallikrein 3 antibody | 1 |
EK0816-ST | Lyophilized recombinant human Kallikrein 3 standard | 20ng/tube×2 |
EK0816-DA | Biotinylated anti- human Kallikrein 3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 551 | 2528 | 8207 | 568 | 2328 | 8686 |
Standard deviation | 36.91 | 156.73 | 648.35 | 47.14 | 144.33 | 773.05 |
CV(%) | 6.7 | 6.2 | 7.9 | 8.3 | 6.2 | 8.9 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.005 | 0.118 | 0.224 | 0.378 | 0.702 | 1.346 | 1.694 | 2.178 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)