产品名称
Human CCN3/NOV ELISA Kit
指标别名
cellular communication network factor 3; IBP-9; IGFBP-9; IGFBP9; NOV; NOVh
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
nephroblastoma overexpressed
文献引用格式
Human CCN3/NOV ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0833)
背景资料
NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0833-CAP | 96-well plate precoated with anti- human NOV antibody | 1 |
EK0833-ST | Lyophilized recombinant human NOV standard | 10ng/tube×2 |
EK0833-DA | Biotinylated anti- human NOV antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 66 | 265 | 820 | 61 | 258 | 754 |
Standard deviation | 2.77 | 16.43 | 37.72 | 3.41 | 19.86 | 45.24 |
CV(%) | 4.2 | 6.2 | 4.6 | 5.6 | 7.7 | 6 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.055 | 0.094 | 0.110 | 0.172 | 0.327 | 0.560 | 1.055 | 1.926 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)