产品名称
	
								
									Human RANKL/TNFSF11 ELISA Kit 
								
							 
														
														
								 指标别名
	
								
																		CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; RANKL; sOdf; TNFSF11; TRANCE								
							 
														
														
								交叉反应
	
								
																		There is no detectable cross-reactivity with other relevant proteins.								
							 
														
								检测范围
	
								
																		78 pg/ml - 5000 pg/ml								
							 
														
														
								基因名全称
	
								
																		tumor necrosis factor (ligand) superfamily, member 11								
							 
														
														
								文献引用格式
	
								
																		Human RANKL/TNFSF11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0842)								
							 
														
								背景资料
	
								
																		Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11(TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor(TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.								
							 
														
														
								试剂盒内容
	
								
																		
| Catalog number | Description | Quantity | 
|---|
| EK0842-CAP | 96-well plate precoated with anti- human TNFSF11 antibody | 1 | 
| EK0842-ST | Lyophilized recombinant human TNFSF11 standard | 10ng/tube×2 | 
| EK0842-DA | Biotinylated anti- human TNFSF11 antibody | 100μl(dilution 1:100) | 
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) | 
| AR1106-1 | Sample diluent buffer | 30 ml | 
| AR1106-2 | Antibody diluent buffer | 12ml | 
| AR1106-3 | ABC diluent buffer | 12ml | 
| AR1104 | TMB color developing agent | 10ml | 
| AR1105 | TMB stop solution | 10ml | 
| PLA-SEA | Adhesive cover | 4 | 
								 
							 
														
								批内差/批间差
	
								
																		
 | Intra-Assay Precision  | Inter-Assay Precision  | 
| Sample | 1 | 2 | 3 | 1 | 2 | 3 | 
| n | 16 | 16 | 16 | 24 | 24 | 24 | 
| Mean(pg/ml) | 115 | 865 | 2242 | 114 | 797 | 2279 | 
| Standard deviation | 5.52 | 53.63 | 105.37 | 5.92 | 58.97 | 116.22 | 
| CV(%) | 4.8 | 6.2 | 4.7 | 5.2 | 7.4 | 5.1 | 
								 
							 
														
								典型数据示例
	
								
																		   | Concentration(pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 
| O.D. | 0.017 | 0.115 | 0.227 | 0.416 | 0.573 | 1.015 | 1.591 | 2.039 | 
								 
							 
														
								保存条件与有效期
	
								
																		Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)								
							 
														
														
														
														
								样本类型
	
								
																		Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)