Human MPO ELISA Kit

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EK0850

96T ￥2480
货期：现货

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Human MPO ELISA Kit standard curve
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产品简介>

产品名称

Human MPO ELISA Kit

规格/价格

96T/2480

指标别名

MPO; myeloperoxidase

免疫原

NSO, A49-S745

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10 pg/ml

检测范围

312 pg/ml - 20000 pg/ml

基因名称

MPO

基因名全称

myeloperoxidase

Uniprot ID

P05164

文献引用格式

Human MPO ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0850)

背景资料

Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697(A49-S745) amino acids with the molecular mass of 80KDa.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0850-CAP	96-well plate precoated with anti- human MPO antibody	1
EK0850-ST	Lyophilized recombinant human MPO standard	20ng/tube×2
EK0850-DA	Biotinylated anti- human MPO antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	580	2272	9558	587	2421	9230
Standard deviation	42.92	154.49	401.43	48.13	191.25	507.65
CV(%)	7.4	6.8	4.2	8.2	7.9	5.5

典型数据示例

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.016	0.130	0.232	0.431	0.720	1.183	1.620	1.916

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

应用范围

ELISA

检验物种

human

检测方法

酶联免疫双抗夹心法

样本类型

Cell Culture Supernatants, cell lysate, Serum, Plasma (heparin, EDTA), Saliva and Urine

Human MPO ELISA Kit

EK0850

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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Human MPO ELISA Kit

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