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Human OX40L/TNFSF4 ELISA Kit

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EK0857

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human OX40L/TNFSF4 ELISA Kit
规格/价格
96T/2480
指标别名
GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40L
免疫原
NSO, Q51-L183
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<10 pg/ml
检测范围
156 pg/ml - 10000 pg/ml
基因名称
TNFSF4
基因名全称
tumor necrosis factor (ligand) superfamily, member 4
Uniprot ID
P23510  
文献引用格式
Human OX40L/TNFSF4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0857)
背景资料
OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
显色时间
20-25分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0857-CAP96-well plate precoated with anti- human TNFSF4 antibody1
EK0857-STLyophilized recombinant human TNFSF4standard10ng/tube×2
EK0857-DABiotinylated anti- human TNFSF4 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2091225464122812774885
Standard deviation8.5651.45306.312.5472.78366.37
CV(%)4.14.26.65.55.77.5
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0010.0790.1710.3410.6061.0931.5192.284
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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