产品名称
Human OX40L/TNFSF4 ELISA Kit
指标别名
GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40L
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
tumor necrosis factor (ligand) superfamily, member 4
文献引用格式
Human OX40L/TNFSF4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0857)
背景资料
OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0857-CAP | 96-well plate precoated with anti- human TNFSF4 antibody | 1 |
EK0857-ST | Lyophilized recombinant human TNFSF4standard | 10ng/tube×2 |
EK0857-DA | Biotinylated anti- human TNFSF4 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 209 | 1225 | 4641 | 228 | 1277 | 4885 |
Standard deviation | 8.56 | 51.45 | 306.3 | 12.54 | 72.78 | 366.37 |
CV(%) | 4.1 | 4.2 | 6.6 | 5.5 | 5.7 | 7.5 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.001 | 0.079 | 0.171 | 0.341 | 0.606 | 1.093 | 1.519 | 2.284 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)