产品名称
Human DKK1 ELISA Kit
指标别名
Dickkopf 1; Dickkopf related protein 1; DKK 1; DKK1; hDkk 1; SK
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
dickkopf WNT signaling pathway inhibitor 1
文献引用格式
Human DKK1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0867)
背景资料
Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0867-CAP | 96-well plate precoated with anti- human DKK1 antibody | 1 |
EK0867-ST | Lyophilized recombinant human DKK1 standard | 10ng/tube×2 |
EK0867-DA | Biotinylated anti- human DKK1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 108 | 660 | 1140 | 103 | 594 | 1134 |
Standard deviation | 5.72 | 47.52 | 66.12 | 6.59 | 43.36 | 82.78 |
CV(%) | 5.3 | 7.2 | 5.8 | 6.4 | 7.3 | 7.3 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.016 | 0.056 | 0.140 | 0.242 | 0.484 | 0.927 | 1.796 | 2.370 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)