产品名称
Human 2B4/CD24 ELISA Kit
指标别名
2B4; CD244; h2B4; NAIL; NKR2B4; SLAMF4
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
CD244 molecule, natural killer cell receptor 2B4
文献引用格式
Human 2B4/CD24 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0926)
背景资料
CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0926-CAP | 96-well plate precoated with anti- human CD244 antibody | 1 |
EK0926-ST | Lyophilized recombinant human CD244 standard | 10ng/tube×2 |
EK0926-DA | Biotinylated anti- human CD244 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 28 | 81 | 453 | 30 | 112 | 469 |
Standard deviation | 1.34 | 4.62 | 20.84 | 1.53 | 6.61 | 30.02 |
CV(%) | 4.8 | 5.7 | 4.6 | 5.1 | 5.9 | 6.4 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.025 | 0.199 | 0.292 | 0.464 | 0.934 | 1.510 | 2.026 | 2.547 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)