产品名称
Human ADAMTS13 ELISA Kit
指标别名
ADAM TS 13; ADAM TS13; ADAMTS 13; ADAMTS13; C9orf8; DKFZp434C2322; FLJ42993; TTP; vWF cleaving protease; vWF CP; VWFCP
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
0.78 ng/ml - 50 ng/ml
基因名全称
ADAM metallopeptidase with thrombospondin type 1 motif, 13
文献引用格式
Human ADAMTS13 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0927)
背景资料
ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0927-CAP | 96-well plate precoated with anti- human ADAMTS13 antibody | 1 |
EK0927-ST | Lyophilized recombinant human ADAMTS13 standard | 50ng/tube×2 |
EK0927-DA | Biotinylated anti- human ADAMTS13 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1395 | 7236 | 20816 | 1322 | 6617 | 20301 |
Standard deviation | 104.62 | 289.44 | 1165.69 | 104.43 | 291.14 | 1197.75 |
CV(%) | 7.5 | 4 | 5.6 | 7.9 | 4.4 | 5.9 |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.051 | 0.101 | 0.134\0.243 | 0.439 | 0.829 | 1.482 | 2.472 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)