Mouse IL12(p40) ELISA Kit

96T/2480

CLMF; CLMF p40; CLMF2; IL 12 subunit p40; IL 12B; IL12B; IL-12B; Interleukin 12 subunit beta; NKSF; NKSF2

Expression system for standard: NS0; Immunogen sequence: M23-S335

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

15.6pg/ml-1000pg/ml

IL12B

Interleukin-12 subunit beta

P43432  

Mouse IL12(p40) ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0932)

Interleukin-12 (IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40 (-/-) and p35 (-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.

Catalog number	Description	Quantity
EK0932-CAP	96-well plate precoated with anti- mouse IL-12(p40) antibody	1
EK0932-ST	Lyophilized recombinant mouse IL-12(p40) standard	10ng/tube×2
EK0932-DA	Biotinylated anti- mouse IL-12(p40) antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	33	233	425	35	253	463
Standard deviation	1.84	13.98	22.52	1.96	17.45	29.63
CV(%)	5.6%	6%	5.3%	5.6%	6.9%	6.4%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.006	0.075	0.151	0.259	0.552	0.929	1.709	2.391

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h