产品名称
Mouse Endothelin ELISA Kit
指标别名
EDN1; EDN2; EDN3; endothelin 1/2/3; ET 1/2/3 Big endothelin 1; ET1; ET2; ET3; HDLCQ7; PPET1; PPET2; PPET3; Preproendothelin 1/2/3
免疫原
A peptide reflecting the target protein in human. Sequence: C53-W73
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
endothelin 1; endothelin 2; endothelin 3
文献引用格式
Mouse Endothelin ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0953)
背景资料
The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0953-CAP | 96-well plate precoated with anti- mouse Endothelin antibody | 1 |
EK0953-ST | Lyophilized recombinant mouse Endothelin standard | 1ng/tube×2 |
EK0953-DA | Biotinylated anti- mouse Endothelin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 19 | 52 | 101 | 18 | 54 | 95 |
Standard deviation | 1.25 | 3.69 | 4.04 | 1.33 | 4.15 | 4.94 |
CV(%) | 6.6% | 7.1% | 4% | 7.4% | 7.7% | 5.2% |
典型数据示例
Concentration(pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
O.D. | 0.024 | 0.054 | 0.10 | 0.262 | 0.502 | 1.112 | 1.754 | 2.410 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
蛋白名全称
Endothelin-1; Endothelin-2; Endothelin-3
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)