Mouse Endothelin ELISA Kit

96T/2480

EDN1; EDN2; EDN3; endothelin 1/2/3; ET 1/2/3 Big endothelin 1; ET1; ET2; ET3; HDLCQ7; PPET1; PPET2; PPET3; Preproendothelin 1/2/3

A peptide reflecting the target protein in human. Sequence: C53-W73

There is no detectable cross-reactivity with other relevant proteins.

<0.5pg/ml

3.9pg/ml-250pg/ml

EDN1/EDN2/EDN3

endothelin 1; endothelin 2; endothelin 3

P22387/P22389/P48299

Mouse Endothelin ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0953)

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

Catalog number	Description	Quantity
EK0953-CAP	96-well plate precoated with anti- mouse Endothelin antibody	1
EK0953-ST	Lyophilized recombinant mouse Endothelin standard	1ng/tube×2
EK0953-DA	Biotinylated anti- mouse Endothelin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	19	52	101	18	54	95
Standard deviation	1.25	3.69	4.04	1.33	4.15	4.94
CV(%)	6.6%	7.1%	4%	7.4%	7.7%	5.2%

Concentration(pg/ml)	0	3.9	7.8	15.6	31.2	62.5	125	250
O.D.	0.024	0.054	0.10	0.262	0.502	1.112	1.754	2.410

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

Endothelin-1; Endothelin-2; Endothelin-3

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h