Human ANGPTL4 ELISA Kit

96T/2480

angiopoietin like 4; Angiopoietin like protein 4; Angiopoietin related protein 4; ANGPTL4; ARP4; FIAF; HFARP; NL2; PGAR; pp1158; PSEC0166; UNQ171/PRO197

NSO, L165-S406

There is no detectable cross-reactivity with other relevant proteins.

<20 pg/ml

625 pg/ml - 40000 pg/ml

ANGPTL4

angiopoietin-like 4

Q9BY76

Human ANGPTL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0960)

Angiopoietin-related protein 4(Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

20-25分钟

Catalog number	Description	Quantity
EK0960-CAP	96-well plate precoated with anti- human ANGPTL4 antibody	1
EK0960-ST	Lyophilized recombinant human ANGPTL4 standard	40ng/tube×2
EK0960-DA	Biotinylated anti- human ANGPTL4 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1114	4583	20920	1120	4790	21489
Standard deviation	73.52	256.64	941.4	79.52	320.93	988.49
CV(%)	6.6	5.6	4.5	7.1	6.7	4.6

Concentration(pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.001	0.201	0.350	0.599	0.929	1.351	1.725	2.196

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h