产品名称
Human Cathepsin S/CTSS ELISA Kit
免疫原
NSO, Q17-I331&S109-I331
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
文献引用格式
Human Cathepsin S/CTSS ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0961)
背景资料
Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization(FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0961-CAP | 96-well plate precoated with anti- human Cathepsin S antibody | 1 |
EK0961-ST | Lyophilized recombinant human Cathepsin S standard | 10ng/tube×2 |
EK0961-DA | Biotinylated anti- human Cathepsin S antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 177 | 404 | 1975 | 188 | 415 | 1910 |
Standard deviation | 10.08 | 19.79 | 126.4 | 11.65 | 26.14 | 154.71 |
CV(%) | 5.7 | 4.9 | 6.4 | 6.2 | 6.3 | 8.1 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.074 | 0.140 | 0.230 | 0.369 | 0.636 | 1.058 | 1.702 | 2.288 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)